Spring is here in the northern hemisphere, which means it's time for the annual American Association of Physical Anthropologists and Society for American Archaeology meetings. Pet sitters and rides to the airport were frantically arranged as nearly everyone in the building has flown to either Austin, Texas or Washington, DC. Another surge in activity was dedicated to getting posters printed in time for either meeting. Academic posters are typically used at conferences and other such meetings to practically and concisely convey information about a project to a fluid and often wide-ranging (age, speciality, language, etc.) audience. Posters are so important to academia that there are dozens and dozens of papers and blog posts/tutorials written about them; some even offer templates. One published in Annals of Medicine and Surgery provides a rather basic guide to preparing an effective academic poster, but we in the Perry Lab have our own preferences that I would like to share with you. Why should you listen to us? Well just check out these two beauties, designed and presented at AAPA2018 by undergrad Audrey Arner and PhD student Maggie Hernandez. Maggie even won a graduate student poster award at PSU's Anthropology Day last week! I can only hope my poster for Evolution 2018 will be as lovely and potent as theirs. Click the images to see a full-scale PDF version, courtesy of Audrey and Maggie. Thanks guys!
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Some of you may remember, last semester I was searching for an undergraduate volunteer to help me out with some wet lab work. After a few interviews and a lot of emails, I've recruited two pretty cool young ladies - one senior, one freshman - to begin instructing this semester :D I am incredibly excited to work with them, and I thought I'd dedicate today's blog post to describing how I'll be training them in our wet lab over the course of Penn State's Spring 2018 semester. Please share your training techniques with me, especially if you notice I'm missing any essential components haha.
I was so excited for today's blog post that I tweeted about it last night when I was leaving work. I also called my mom; she didn't answer because she was out with a friend, but it was worth it for the extremely fitting text exchange we had afterwards.
I'd like to share with you today some of our experiences from last night. Why? Because sometimes your career in a research field will lead you down some pretty bizarre paths. While your paths might not necessarily be as smelly as the one we took last night, or the one I took in my Garage Lab, you come to appreciate how many unique opportunities you'll have as a research scientist. If nothing else, they all will make for incredible stories one day.
Warning: A lot of the images that follow in this blog post are just gross, and I realize that some of you (like the bear in the gif below) might not want to see photos of bear guts and contents. Therefore, most pictures are shared as links you can click on at your own risk.
Some of you might remember my Panama posts from a few weeks ago (if you do, thanks for reading my blog!). One of those posts focused on my work in Bocas del Toro, which I've been daydreaming about ever since the temperature dropped in State College. I went to Bocas to collect conch shells and bring them back to PSU so I could attempt to extract DNA from them for my dissertation work.
Needless to say, even though all of the shells were at least quadruple-bagged, all of my clothes went into the wash as soon as I got home and the snails needed to be stored in my garage until I was ready to work with them. I couldn't inflict this kind of stench on the lovely people in the Anthropology building where I work.
Day 1 - 2017-09-02 |
This post is going to be all about my week in Bocas del Toro (BDT). BDT Tip #1: Travel light! You literally have to if you're flying up from Panama City because there is a 14kg weight limit per passenger. Another tip for those who fly: be patient. As quoted from a member of Team Bocas [see end of post], Air Panama tends to run more like a bus station than an airport. |
This week I found myself needing to make a map that depicted the current range of Propithecus verreauxi lemur populations throughout southwestern Madagascar. You know, as one does.
Lucky for me, the folks over at the International Union for Conservation of Nature (IUCN) maintains a Red List of Threatened Species, which evaluates the conservation status of species all around the world. You can search a species, and the IUCN database will explain how the species is classified into one of the following categories: Not Evaluated, Data Deficient, Least Concern, Near Threatened, Vulnerable, Endangered, Critically Endangered, Extinct in the Wild, or Extinct. The assessment information is listed in a table of publications from which they have acquired data, including the current species range (click “View Map”). |
I downloaded the Spatial Data for P. verreauxi (see bottom left), and had to figure out how to add this “shape” data to a map of Madagascar. After trying out a bunch of different packages, I ended up going with a basic maps format (still working on getting a nice looking ggmap). I’ve managed do two things so far: plot the species range to 1) a full country map of Madagascar [bottom middle] and 2) to a “zoomed in” inset of Madagascar that highlights how close the red and black sites are to each other [bottom right]. |
Ideally the large map of Madagascar will be inside that white inset on the “zoomed in” map, but I’ve yet to make the add = True argument actually do what it’s supposed to. Scroll all the way down to see the code I used. Anyone have some helpful hints?
Also, if it makes you sad that these gorgeous sifakas are on the endangered species list and you would like to help a conservation effort in some way, I would like to recommend beginning your lemur aid efforts by checking out LemurLove. They are some good people just trying spread the love for lemurs :) Cheers!
Before I get too swept up in the world of 3D data, I’d like to ask how y’all feel about this new feature I’ve added to the blog ---------------------------------------------------->
I noticed last week that I lack a search box, and this would not be tolerated. I had to make do with what was available (other than paying for a fancier version of this kind of blog to acquire a search feature). I found this nifty "Accordion" application, and would love to know if you think this is an adequate replacement for a real-deal search feature, please and thanks :)
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Now, back to business. I left for Madagascar almost exactly one year ago. We spent the second half of the three-week trip traveling throughout the southwest and collecting morphological data from an osteological collection at Beza Mahafaly. The first half of our visit (and today’s blog post) was dedicated to exploring the osteological collections at the University of Antananarivo.
As I’ve mentioned previously, we had this fancy new Artec Space Spider 3D surface scanner and couldn’t wait to start using it. Since our time was limited, we focused our scanning efforts on especially rare specimens, skulls from a bunch of extinct lemur species, and anything else that was super cool (AKA, things that PJ got especially excited about scanning/photographing). In my spare time, I’ve been cleaning and assembling the scan data for each specimen into 3D objects, and we’d love to share them with you (if you have an interest in that sort of thing). |
I will be uploading these post-processed 3D objects to MorphoSource, which is essentially a free virtual museum for 3D data that's primarily funded by Duke University. Our repository currently contains a collection of Daubentonia robusta (giant aye-aye) elements, a full Daubentonia madagascariensis (aye-aye) hand, and an Archaeoindris fontoynontii femur (see right; 10-inch diameter turntable for scale). I will eventually be uploading subfossil elements from a good portion of the extinct lemur species, along with a few modern ones later on, but if you are particularly interested in seeing this 3D data sooner rather than later please feel free to contact me or PJ. No export permits required! Cheers :) |
So remember my last Research blog update where I talked about the Perry Lab endeavors to find the best possible way to smush lice for DNA extractions? Well I'm at it again!
As I've previously mentioned, there are three main tasks to complete when beginning a new DNA sequencing project: 1) get the DNA out of your sample, 2) prepare your DNA libraries for sequencing, and 3) do both 1 and 2 in a cost-effective manner. I dedicated yesterday to step 2 of this process.
A DNA library is pretty much what it sounds like: all of the genomic DNA from an individual sorted and organized in such a way that it is more amenable to sequencing. A good portion of genomic sequencing is performed on machines made by Illumina, so naturally they've also created kits for preparing your DNA libraries. The Perry Lab has had success with these kits, but recall point 3 from above: costs. Kits are generally more expensive than what are referred to as "in-house" protocols because you can typically buy the same reagents you would use in the kit in bulk for a lower price. Enter Matthias Meyer and Martin Kircher. |
Meyer & Kircher's protocol Illumina sequencing library preparation for highly multiplexed target capture and sequencing (M&K) has become a Perry Lab standard. M&K is nearly identical to Illumina's equivalent library prep protocol (see above figure), with the only real difference being the making of master mixes. The nice thing about kits is that every reagent you'll need is already prepared for you in a proprietary concoction, so you can just grab and go with each step. For M&K, you have to put together the same ingredients that are found in the ready-made mixes. This adds a bit of time, but in the end the procedure is a bit more controlled by the research group, which is handy if protocols need to be adapted for any particular reason (ours is ancient DNA).
The really cool thing about both of these protocols is the use of magnetic beads to directly manipulate the DNA strands in their solution (see depiction at left for Agencourt AMpure XP beads). We make our own Solid Phase Reversible Immobilization (SPRI) beads in-house for use in both our ancient and modern lab spaces. The sort of gross looking tube of brown to the left contains these teeny tiny SPRI beads that we can use to "clean" our DNA throughout the library prep process. We can also manipulate the ratio of beads to DNA to select DNA fragments of whatever length we are targeting - in DNA sequencing, length matters. (For those who are curious, the tube is wrapped in foil because the beads are photosensitive and will degrade if exposed to too much light.) |
This past week and a half of life in the Perry lab has been rather exciting. We've recently welcomed a new Ph.D.-track Biology grad student, Cory Henderson, as well as a visiting undergrad student from Howard University, NaTazah O'Neil, who will spend the summer with the Perry lab to get some bioinformatics experience. I've been working with Cory for the past few days on a rather interesting task: how best to extract the DNA from a body louse (plural = lice). |
Cory is attempting to get lice DNA because he is broadly interested in the evolutionary relationship between humans and their parasites. PJ and Cory will be working closely with Professor John Marshall Clark's lab up at UMass Amherst, where they maintain colonies of head and body lice. For those who are unfamiliar, lice are parasitic insects that feed on human blood. They can infect any human population worldwide, and are easily transmitted in crowded living conditions with poor hygiene. For more info, visit the CDC's website.
One of the first steps to beginning a new wet lab experiment is optimizing your protocols for your lab space, or vice versa. I say vice versa because sometimes the best way to ensure the success of your protocol is to buy a fancy new piece of equipment - one recent example from the Perry lab was a new speed vac concentrator :) In the world of DNA sequencing, you have three essential components to figure out in the first stages of your project: 1) how to get the DNA out of your sample, 2) how to prepare your DNA libraries for sequencing, and 3) how to do both 1 and 2 in a cost-effective manner. Dr. Clark's lab mailed us 50 body lice last week so we could begin optimizing Cory's DNA extraction protocols. |
Most DNA extraction protocols for tissues will have you prepare said tissue by grinding it up in a buffer solution (AKA "homogenizing" it) then digesting it in proteinase K (which breaks down proteins to release more DNA) for a specified amount of time. I have previously ground up a few tapeworms in the Perry modern lab with plastic pestles, and we also have a fancy electronic homogenizer (that I nicknamed the nose-hair trimmer) that essentially makes a smoothie of your tissue. But Dr. Clark's lab's favorite homogenizing tool is this glass-glass homogenizer.
Since we really wanted to try a few extractions this week and Cory's leaving for a conference in Zanzibar tomorrow, we decided to test our plastic pestles and electronic homogenizer to see how they'd get the job done. Pictured on the left are Cory's lice. Using sterile lab techniques, he would fish one out, wash it off, then add it to some buffer in a neatly labeled microtube for homogenization. |
Long story short, our fancy homogenizer failed completely and our pestles worked sort of okay. The lice were small enough that they wouldn't get sucked into the homogenizer; they'd just spin around in the buffer solution, which eventually became so foamy that we couldn't even tell if the bug was getting ground up or not. The plastic pestles were a bit better at breaking up the lice, but weren't as effective as we hoped. So here we are, trying to get these silly little dead bugs to cooperate with our various grinding techniques, when another grad student in the Anth department, Kelsey Kjosness, saved the day with a scalpel. Simply cutting up the louse a bit before trying to pulverize it with a pestle yielded much more DNA than mashing alone. Sometimes old school = best school.
Cory will continue refining his louse extraction techniques upon his return from Zanzibar, probably with the help of a glass-glass homogenizer or two. While the first stages of a new project may seem tedious, it is also a rather exciting time as a researcher. It is so completely satisfying to finally have "this is the best way to do this step" checked off on your project to-do list. I hope to be checking off my own extraction to-do list rather soon, and I will keep y'all updated on that! Cheers :)
This post has been a while coming, but it's here at last! After going through the processes so beautifully illustrated in this gif, my first 1st-author research article has officially been published :D An evolutionary medicine perspective on Neandertal extinction is available online through the Journal of Human Evolution (yay for another addition to my "Publications" page :D ). |
As I've mentioned on my "Research" page, I began my work on this project during my first few months as an official member of PJ's lab. I had come into the lab with absolutely zero bioinformatics experience, and what better way to learn than to try a little mini project of my own? This analysis was based on the work (and genomes) Castellano et al published in their 2014 PNAS paper Patterns of coding variation in the complete exomes of three Neandertals. I've decided to use this blog post to provide a bit of background genetics information that you'd probably appreciate if you're unfamiliar with the field but would still like to take a stab at reading the paper just because you like me :)
Our genetic information is carried in our DNA [deoxyribonucleic acid], which is composed of the nucleotides Adenine (A), Cytosine (C), Guanine (G), and Thymine (T) [see image above left]. During the interphase portion of the cell cycle (ie, when cell growth and development takes place), our genetic information is transcribed into RNA [ribonucleic acid]. RNA is composed of the same nucleotides as DNA, except a Uracil (U) takes the place of the thymines. Then this RNA is translated into the amino acid sequence of a protein. Proteins are made up of hundreds of amino acids, and there are only 20 amino acids to choose from [see image above right]. Here's a neat video that draws out the process for you.
Proteins are often called the "workhorses of the cell" because they are largely responsible for the structure, function, and regulation of our tissues and organs. The motor protein featured on the left is an excellent example of a protein at work in our cells. It is therefore essential that transcription and translation correctly assemble these proteins, otherwise our bodies won't work the way they should. |
recap: DNA --> RNA --> Amino Acids --> Proteins --> US
I drew out a hypothetical example of a segment of DNA going through transcription and translation to end up as three amino acids: Cysteine, Valine, and Leucine. In certain individual spots of the human nuclear genome, there are these things called SNPs [Single Nucleotide Polymorphisms]. As the name kind of suggests, a single nucleotide [A, C, G, T] can take multiple forms within the individuals of a population.
So let's say your genome has the above DNA sequence, and my genome has the one pictured here. At the green highlighted spot, my DNA will transcribe a cytosine into my RNA where yours has a guanine. This change doesn't actually change the protein that is formed when my RNA is translated, so our proteins will probably work the same way. This is called a synonymous SNP. |
As you might have guessed, sometimes these SNPs might cause our RNA to be translated into a different amino acid. This is called a nonsynonymous SNP, and is depicted on the left. If we have genomic data available for a population of organisms (in my case, modern humans and Neandertals), we can use a program called PolyPhen-2 to predict whether or not these nonsynonymous SNPs will functionally change ("damage") the structure of the protein. |
Castellano's group generated a dataset in which they compared the number of potentially damaging nonsynonymous SNPs in Neandertal genomes to the number found in modern human genomes. They published evidence that Neandertals had much lower genetic diversity than modern human populations from Africa, Europe, and Asia, and that Neandertals had a nearly 50:50 ratio of damaging to non-damaging nonsynonmous SNPs (a much higher proportion when compared to the modern human populations). My analysis replicated the one conducted by Castellano et al, and went a bit further by assessing these same kinds of patterns within certain gene families that are associated with innate immunity.
I gave a talk about this project at AAPA 2016 and have provided the slides here for your perusing pleasure. Otherwise check out the paper to find out more, especially since we've updated a bunch of things since we uploaded our initial bioRxiv preprint :) Enjoy!
All Research Updates
Visit to the Duke Lemur Center - July 2015 [Parts 1, 2, 3, 4, 5]
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"Mada-hoo-ah?" "No, not hoo-ah. As-car."
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Antananarivo - Week 1
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My very first first-author publication!!!
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Beza Mahafaly - Week 2.5-3
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Ancient DNA Lab
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AAPA 2017 + UpGoer Five Symposium
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My latest publication :D Let's talk about SNPs
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Protocol optimization: How to grind up lice
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Protocol optimization: I am attracted to good magnets
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MorphoSource Lemur Surface Scan Repository
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Making a Species Range Map in R
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Bocas del Toro - Panama [Part 3]
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Garage Lab
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Dirty Jobs: Science Edition
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Training My Undergrad Volunteer Research Assistants
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Preparing Pretty Poster Presentations
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All About Aye-ayes
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Evolution - Montpellier 2018
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Oh The Places She's Gone...For Science
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Making Things Easier For Yourself: A Love Letter To Technology
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Say Hi To Perry Lab People At AAPA 2019
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Evolution - Providence 2019
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One-Slide Research Summaries
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Productivity and Mental Health
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"Mada-hoo-ah?" "No, not hoo-ah. As-car."
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Antananarivo - Week 1
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My very first first-author publication!!!
-----------------------
Beza Mahafaly - Week 2.5-3
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Ancient DNA Lab
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AAPA 2017 + UpGoer Five Symposium
-----------------------
My latest publication :D Let's talk about SNPs
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Protocol optimization: How to grind up lice
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Protocol optimization: I am attracted to good magnets
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MorphoSource Lemur Surface Scan Repository
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Making a Species Range Map in R
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Bocas del Toro - Panama [Part 3]
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Garage Lab
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Dirty Jobs: Science Edition
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Training My Undergrad Volunteer Research Assistants
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Preparing Pretty Poster Presentations
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All About Aye-ayes
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Evolution - Montpellier 2018
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Oh The Places She's Gone...For Science
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Making Things Easier For Yourself: A Love Letter To Technology
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Say Hi To Perry Lab People At AAPA 2019
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Evolution - Providence 2019
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One-Slide Research Summaries
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Productivity and Mental Health
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All Sci Comm Updates
It begins with being taught how to teach...
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Mueller: Semi-new and definitely improved
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Sully’s Top Three Teaching Tips
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Sylly Week & Survey
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www.online-resources-i-use-when-teaching.com
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Journal Club
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Oral Presentations
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Teaching --> Sci Comm
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PA Junior Academy of Science
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Making Things Easier for Yourself: Research Edition
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Neandertal R Stuffs
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I submitted a grant proposal that was only 140 characters long…
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Perry Lab Meeting Up Goer 5
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Preparing for Fieldwork [Panama, Part 2]
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STRI Tupper Seminar - Panama [Part 5]
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COMPS! 2/3 of the way to Ph.D.
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Research Opportunity for PSU Undergraduate Student
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Sci Comm Spotlight: Skype A Scientist
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Sci Comm Spotlight: March Mammal Madness
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PostDoc Appreciation
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Mueller: Semi-new and definitely improved
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Sully’s Top Three Teaching Tips
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Sylly Week & Survey
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www.online-resources-i-use-when-teaching.com
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Journal Club
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Oral Presentations
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Teaching --> Sci Comm
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PA Junior Academy of Science
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Making Things Easier for Yourself: Research Edition
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Neandertal R Stuffs
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I submitted a grant proposal that was only 140 characters long…
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Perry Lab Meeting Up Goer 5
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Preparing for Fieldwork [Panama, Part 2]
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STRI Tupper Seminar - Panama [Part 5]
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COMPS! 2/3 of the way to Ph.D.
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Research Opportunity for PSU Undergraduate Student
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Sci Comm Spotlight: Skype A Scientist
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Sci Comm Spotlight: March Mammal Madness
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PostDoc Appreciation
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All Life
Updates
Updates
1st Rugelach Attempt
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Vacation, bread, and new-ish cat tree
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New Year, New Blog Goals
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New Year, New Blog Goals [Take 2]
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How I Decided Where to Go for My Education – High School, Undergrad, and Grad School
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Lab Meeting Bakes
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Not Your Typical International Women’s Day Post
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Yeast is Beast: Microbiology in Your Kitchen
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Work & Play in New Orleans
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Dan’s Improved Eyes
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The Saga of Poopy Moo
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We’re Shipping Up to Boston, Woah-oh-oh
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Reducing Our Waste-Line
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What Do You Want to Eat This Week?
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Panama! [Part 1]
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Mini Vacation with Momma - Panama [Part 4]
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Let Them Eat Cake [Part 1]
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2017 Fall Break
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Why I Never Liked The Color Pink
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Let Them Eat Cake [Part 2]
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Vermicomposting
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I'm Going To France!
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Recent Recipes: BA's Best, Some Standbys, and French Macaron
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Applying to Grad School - Q&A
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Hello 2019!
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Cookbook Review: The Barefoot Contessa Cookbook, Ina Garten
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Recent Recipes: Misfit Produce, Valentine's Traditions, And New Favorites
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Struggles Of Being A Scientist As A Woman/Person With Lady Bits
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My Dad's Melanoma: Biopsies and Surgeries
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Happy Birthday To Me / Q&A-A-Day
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Recent Recipes: Our Favorite Fries
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Vacation, bread, and new-ish cat tree
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New Year, New Blog Goals
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New Year, New Blog Goals [Take 2]
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How I Decided Where to Go for My Education – High School, Undergrad, and Grad School
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Lab Meeting Bakes
-----------------------
Not Your Typical International Women’s Day Post
-----------------------
Yeast is Beast: Microbiology in Your Kitchen
-----------------------
Work & Play in New Orleans
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Dan’s Improved Eyes
-----------------------
The Saga of Poopy Moo
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We’re Shipping Up to Boston, Woah-oh-oh
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Reducing Our Waste-Line
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What Do You Want to Eat This Week?
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Panama! [Part 1]
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Mini Vacation with Momma - Panama [Part 4]
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Let Them Eat Cake [Part 1]
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2017 Fall Break
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Why I Never Liked The Color Pink
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Let Them Eat Cake [Part 2]
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Vermicomposting
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I'm Going To France!
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Recent Recipes: BA's Best, Some Standbys, and French Macaron
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Applying to Grad School - Q&A
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Hello 2019!
-----------------------
Cookbook Review: The Barefoot Contessa Cookbook, Ina Garten
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Recent Recipes: Misfit Produce, Valentine's Traditions, And New Favorites
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Struggles Of Being A Scientist As A Woman/Person With Lady Bits
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My Dad's Melanoma: Biopsies and Surgeries
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Happy Birthday To Me / Q&A-A-Day
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Recent Recipes: Our Favorite Fries
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Dry Lab
Antananarivo – Week 1
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My latest publication :D Let's talk about SNPs
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Making Things Easier for Yourself: Research Edition
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Neandertal R Stuffs
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MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
-----------------------
My latest publication :D Let's talk about SNPs
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Making Things Easier for Yourself: Research Edition
-----------------------
Neandertal R Stuffs
-----------------------
MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
Education
Penn State University
Applying to Grad School - Q&A
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Mueller: Semi-new and Definitely Improved
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COMPS! 2/3 of the way to Ph.D.
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Mueller: Semi-new and Definitely Improved
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COMPS! 2/3 of the way to Ph.D.
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Family
Vacation, bread, and new-ish cat tree
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Not Your Typical International Women’s Day Post
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Work & Play in New Orleans
-----------------------
Dan’s Improved Eyes
-----------------------
The Saga of Poopy Moo
-----------------------
We’re Shipping Up to Boston, Woah-oh-oh
-----------------------
Reducing Our Waste-line
-----------------------
Mini Vacation with Momma - Panama [Part 4]
-----------------------
Let Them Eat Cake [Part 1]
-----------------------
2017 Fall Break
-----------------------
Let Them Eat Cake [Part 2]
-----------------------
Recent Recipes: BA's Best, Some Standbys, and French Macaron
-----------------------
-----------------------
Not Your Typical International Women’s Day Post
-----------------------
Work & Play in New Orleans
-----------------------
Dan’s Improved Eyes
-----------------------
The Saga of Poopy Moo
-----------------------
We’re Shipping Up to Boston, Woah-oh-oh
-----------------------
Reducing Our Waste-line
-----------------------
Mini Vacation with Momma - Panama [Part 4]
-----------------------
Let Them Eat Cake [Part 1]
-----------------------
2017 Fall Break
-----------------------
Let Them Eat Cake [Part 2]
-----------------------
Recent Recipes: BA's Best, Some Standbys, and French Macaron
-----------------------
Food
1st Rugelach Attempt
-----------------------
Vacation, bread, and new-ish cat tree
-----------------------
Lab Meeting Bakes
-----------------------
Yeast is Beast: Microbiology in Your Kitchen
-----------------------
Work & Play in New Orleans
-----------------------
Dan’s Improved Eyes
-----------------------
Reducing Our Waste-Line
-----------------------
What do You Want to Eat This Week?
-----------------------
Mini Vacation with Momma – Panama [Part 4]
-----------------------
Let Them Eat Cake [Part 1]
-----------------------
2017 Fall Break
-----------------------
Let Them Eat Cake [Part 2]
-----------------------
Vermicomposting
-----------------------
Recent Recipes: BA's Best, Some Standbys, and French Macaron
-----------------------
-----------------------
Vacation, bread, and new-ish cat tree
-----------------------
Lab Meeting Bakes
-----------------------
Yeast is Beast: Microbiology in Your Kitchen
-----------------------
Work & Play in New Orleans
-----------------------
Dan’s Improved Eyes
-----------------------
Reducing Our Waste-Line
-----------------------
What do You Want to Eat This Week?
-----------------------
Mini Vacation with Momma – Panama [Part 4]
-----------------------
Let Them Eat Cake [Part 1]
-----------------------
2017 Fall Break
-----------------------
Let Them Eat Cake [Part 2]
-----------------------
Vermicomposting
-----------------------
Recent Recipes: BA's Best, Some Standbys, and French Macaron
-----------------------
Friends
Protocol Optimization: How to Grind Up Lice
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We’re Shipping Up to Boston, Woah-oh-oh
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Bocas del Toro – Panama [Part 3]
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All About Aye-ayes
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Evolution - Montpellier 2018
-----------------------
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We’re Shipping Up to Boston, Woah-oh-oh
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Bocas del Toro – Panama [Part 3]
-----------------------
All About Aye-ayes
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Evolution - Montpellier 2018
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Me
New Year, New Blog Goals and [Take 2]
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How I Decided Where to Go for My Education - High School, Undergrad, and Grad School
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Why I Never Liked The Color Pink
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Applying to Grad School - Q&A
-----------------------
Hello 2019!
-----------------------
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How I Decided Where to Go for My Education - High School, Undergrad, and Grad School
-----------------------
Why I Never Liked The Color Pink
-----------------------
Applying to Grad School - Q&A
-----------------------
Hello 2019!
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Perry lab
Ancient DNA Lab
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Protocol Optimization: How to Grind Up Lice
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Protocol Optimization: I am Attracted to Good Magnets
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MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
Perry Lab Meeting Up Goer 5
-----------------------
Dirty Jobs: Science Edition
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
Preparing Pretty Poster Presentations
-----------------------
All About Aye-ayes
-----------------------
-----------------------
Protocol Optimization: How to Grind Up Lice
-----------------------
Protocol Optimization: I am Attracted to Good Magnets
-----------------------
MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
Perry Lab Meeting Up Goer 5
-----------------------
Dirty Jobs: Science Edition
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
Preparing Pretty Poster Presentations
-----------------------
All About Aye-ayes
-----------------------
Publications
My Very First First-Author Publication!!!
-----------------------
My Latest Publication :D Let’s Talk about SNPs
-----------------------
Neandertal R Stuffs
-----------------------
All About Aye-ayes
-----------------------
-----------------------
My Latest Publication :D Let’s Talk about SNPs
-----------------------
Neandertal R Stuffs
-----------------------
All About Aye-ayes
-----------------------
Sci Comm
Journal Club
-----------------------
Oral Presentations
-----------------------
Teaching --> Sci Comm
-----------------------
PA Junior Academy of Science
-----------------------
Making Things Easier for Yourself: Research Edition
-----------------------
AAPA 2017 + Up Goer Five Symposium
-----------------------
Perry Lab Meeting Up Goer 5
-----------------------
I submitted a grant proposal that was only 140 characters long…
-----------------------
STRI Tupper Seminar - Panama [Part 5]
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COMPS! 2/3 of the way to Ph.D.
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Preparing Pretty Poster Presentations
-----------------------
Sci Comm Spotlight: Skype A Scientist
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Evolution - Montpellier 2018
-----------------------
-----------------------
Oral Presentations
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Teaching --> Sci Comm
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PA Junior Academy of Science
-----------------------
Making Things Easier for Yourself: Research Edition
-----------------------
AAPA 2017 + Up Goer Five Symposium
-----------------------
Perry Lab Meeting Up Goer 5
-----------------------
I submitted a grant proposal that was only 140 characters long…
-----------------------
STRI Tupper Seminar - Panama [Part 5]
-----------------------
COMPS! 2/3 of the way to Ph.D.
-----------------------
Preparing Pretty Poster Presentations
-----------------------
Sci Comm Spotlight: Skype A Scientist
-----------------------
Evolution - Montpellier 2018
-----------------------
Sully Asks A Scientist
Undergrads
Audrey Arner
Grad Students
Daniel T. Schussheim
Margarita Hernandez
Catherine Herzog
Tina Lasisi
Kate M. Lesciotto
Lily Doershuk
Julie D. White
Post Docs
Stephanie Marciniak
Kathleen Grogan
Kathryn G. Turner
PIs
Technicians
Audrey Arner
Grad Students
Daniel T. Schussheim
Margarita Hernandez
Catherine Herzog
Tina Lasisi
Kate M. Lesciotto
Lily Doershuk
Julie D. White
Post Docs
Stephanie Marciniak
Kathleen Grogan
Kathryn G. Turner
PIs
Technicians
Teaching
It begins with being taught how to teach...
-----------------------
Sully’s Top Three Teaching Tips
-----------------------
Sylly Week & Survey
-----------------------
Online Resources I Use When Teaching
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
-----------------------
Sully’s Top Three Teaching Tips
-----------------------
Sylly Week & Survey
-----------------------
Online Resources I Use When Teaching
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
Travels
Vacation, bread, and new-ish cat tree
-----------------------
Visit to the Duke Lemur Center - July 2015 [Parts 1, 2, 3, 4, 5]
-----------------------
“Mada-hoo-ah?” “No, not hoo-ah. As-car.”
-----------------------
Antananarivo - Week 1
-----------------------
Beza Mahafaly - Week 2.5-3
-----------------------
AAPA 2017 + Up Goer Five Symposium
-----------------------
Work & Play in New Orleans
-----------------------
We’re Shipping Up to Boston, Woah-oh-oh
-----------------------
Panama! [Part 1]
-----------------------
Preparing for Fieldwork - Panama [Part 2]
-----------------------
Bocas del Toro - Panama [Part 3]
-----------------------
Mini Vacation with Momma - Panama [Part 4]
-----------------------
STRI Tupper Seminar - Panama [Part 5]
-----------------------
2017 Fall Break
-----------------------
I'm Going To France!
-----------------------
Evolution - Montpellier 2018
-----------------------
-----------------------
Visit to the Duke Lemur Center - July 2015 [Parts 1, 2, 3, 4, 5]
-----------------------
“Mada-hoo-ah?” “No, not hoo-ah. As-car.”
-----------------------
Antananarivo - Week 1
-----------------------
Beza Mahafaly - Week 2.5-3
-----------------------
AAPA 2017 + Up Goer Five Symposium
-----------------------
Work & Play in New Orleans
-----------------------
We’re Shipping Up to Boston, Woah-oh-oh
-----------------------
Panama! [Part 1]
-----------------------
Preparing for Fieldwork - Panama [Part 2]
-----------------------
Bocas del Toro - Panama [Part 3]
-----------------------
Mini Vacation with Momma - Panama [Part 4]
-----------------------
STRI Tupper Seminar - Panama [Part 5]
-----------------------
2017 Fall Break
-----------------------
I'm Going To France!
-----------------------
Evolution - Montpellier 2018
-----------------------
Wet Lab
Ancient DNA Lab
-----------------------
Making Things Easier for Yourself: Research Edition
-----------------------
Protocol Optimization: How to Grind Up Lice
-----------------------
Protocol Optimization: I am Attracted to Good Magnets
-----------------------
Garage Lab
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
Evolution - Montpellier 2018
-----------------------
-----------------------
Making Things Easier for Yourself: Research Edition
-----------------------
Protocol Optimization: How to Grind Up Lice
-----------------------
Protocol Optimization: I am Attracted to Good Magnets
-----------------------
Garage Lab
-----------------------
Training My Undergrad Volunteer Research Assistants
-----------------------
Evolution - Montpellier 2018
-----------------------
Wildlife
Visit to the Duke Lemur Center - July 2015 [Parts 1, 2, 3, 4, 5]
-----------------------
Beza Mahafaly - Week 2.5-3
-----------------------
Protocol Optimization: How to Grind Up Lice
-----------------------
MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
Panama! [Part 1]
-----------------------
Bocas del Toro – Panama [Part 3]
-----------------------
Mini Vacation with Momma – Panama [Part 4]
-----------------------
STRI Tupper Seminar – Panama [Part 5]
-----------------------
Garage Lab
-----------------------
All About Aye-ayes
-----------------------
Evolution - Montpellier 2018
-----------------------
-----------------------
Beza Mahafaly - Week 2.5-3
-----------------------
Protocol Optimization: How to Grind Up Lice
-----------------------
MorphoSource Lemur Surface Scan Repository
-----------------------
Making a Species Range Map in R
-----------------------
Panama! [Part 1]
-----------------------
Bocas del Toro – Panama [Part 3]
-----------------------
Mini Vacation with Momma – Panama [Part 4]
-----------------------
STRI Tupper Seminar – Panama [Part 5]
-----------------------
Garage Lab
-----------------------
All About Aye-ayes
-----------------------
Evolution - Montpellier 2018
-----------------------
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